pstBA [2019-09-02 12:53:28]

phosphate [SW|ABC transporter] (ATP-binding protein)

locus: BSU_24960

pI: 7.90

mw: 29.85 kDa

protein length: 269 aa

gene length: 810 bp

function: high-affinity phosphate uptake

product: phosphate [SW|ABC transporter] (ATP-binding protein)

essential: no

ec: 3.6.3.27

synonyms: pstB,yqgJ,yzmE

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.6|Uptake of ions]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,578,003 2,578,812

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + phosphate --> ADP + H⁺ + 2 phosphate (according to UniProt)

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|468BEA9EAEA589D683DB79B2C689A5C6A22AB67F|GlnQ], [protein|6882EF93EC82872B25C88104F62D6603D2514890|ArtR], [protein|B24763A732111026D21C828FF9BE73FB22A123CF|TcyN], [protein|B453986691E1231A1C565D469A2A60EBFF2F6BD3|TcyC], [protein|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|YxeO], [protein|0D5BC94E21D51373A50D61CE3D0895B3DB28F6B3|PstBB]

Structure

• [PDB|2OLJ] (from Geobacillus stearothermophilus, 37% identity)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|BA45631351E3C9C140D15F620D7B7AC143C04932|pstS]-[gene|8BC7CCE9EBD899912ED7C72CA04FE82B21516F77|pstC]-[gene|5CE86C6768A4EF313FB5DBC270AE2A773EA58E81|pstA]-[gene|434BCED6953BE07D592AC229264C5AD0D04CBAF6|pstBA]-[gene|0D5BC94E21D51373A50D61CE3D0895B3DB28F6B3|pstBB]
  description: [Pubmed|9098050]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9098050], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]-P, [PubMed|9098050,9593301], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

  regulation

  • expressed under conditions of phosphate limitation (5000-fold induced) ([protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050,9593301 PubMed]

  additional information

  • mRNA processing between [gene|BA45631351E3C9C140D15F620D7B7AC143C04932|pstS] and [gene|8BC7CCE9EBD899912ED7C72CA04FE82B21516F77|pstC] to maintain higher concentrations of [protein|BA45631351E3C9C140D15F620D7B7AC143C04932|PstS] relative to other components of the [SW|ABC transporter] [PubMed|15289558]

Biological materials

Mutant

• MGNA-C440 (yqgJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2438 NBRP B. subtilis, Japan]
• BKE24960 ([gene|434BCED6953BE07D592AC229264C5AD0D04CBAF6|pstBA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTTCGCCTCTTTCC, downstream forward: _UP4_GGGAAATTCGGATAGGAGGG
• BKK24960 ([gene|434BCED6953BE07D592AC229264C5AD0D04CBAF6|pstBA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTTCGCCTCTTTCC, downstream forward: _UP4_GGGAAATTCGGATAGGAGGG

labs

• [SW|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]

References

• 15289558,10092453,12897025,10913081,9098050,9098050,9593301